Bentzi Gopstein, head of extremist group Lehava invited the illegal settlers to take part in the event which will be held later this month and mark it by demolishing the Dome of the Rock in the occupied East Jerusalem al-Quds.
He shared a photo on social media that showed a bulldozer beside the Dome of Rock which is situated in the Al-Aqsa Mosque Compound.
"We will come to demolish the Dome of the Rock," read the photo's caption.
The march is due to take place on 28 May.
Calls for demolition of the holy site are not new as it has been repeated in the past decades. Such calls have been strongly condemned by the Palestinian people who have warned Zionists of any provocation in the Al-Aqsa Mosque.
The flag march of last year led to 11-day Israeli aggression on Gaza which started after Palestinian resistance launched some rockers toward occupied territories in response to violations committed by the settlers in the march.